![]() ![]() The only thing different from the paper (in flowjo settings) is that the Arcsinh "Augment" value isn't in FlowJo X (at least thats what the rep told me) and they did their analysis on cytobank but I don't think its that. If I do NC1/2 vs a parameter, I can easily pick out a positive population, but I want to be to look at more than one maker at a time on biaxial plots. Everything is on the 0 when I look at two parameters at once. My concern is the lack of "clouds" in my data. I have the scale set to arcsinh as in the Bendall paper. Basically, my main problem is I can't recapitulate the data that I've seen on here and in the Bendall Science paper. ![]() We are looking at healthy donor human PBMCs thawed from LN2. I am trailblazing these experiments at our university so you guys are my support! Thank you in advance. So I've been trying to analyze some data that we just got off the CyTOF and am having some difficulties. ![]()
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